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Title: | DNA binding and cleavage studies of two palladium(II) saccharinate complexes with terpyridine |
Authors: | Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Anabilim Dalı. 0000-0002-2849-3332 0000-0002-2717-2430 İçsel, Ceyda Yılmaz, Veysel Turan L-7238-2018 AAI-3342-2021 7006269202 55551960400 |
Keywords: | Biochemistry & molecular biology Cell biology Genetics & heredity Cells in-vitro Platinum(II) complexes Thermal-properties Nucleic-acids Metallointercalation reagents Ruthenium(II) complexes Deoxyribonucleic-acid Crystal-structures Ethidium bromide Metal-complexes Vesicular stomatitis virus |
Issue Date: | Apr-2013 |
Publisher: | Mary Ann Liebert |
Citation: | İçsel, C. ve Yılmaz, V. T. (2011). "DNA binding and cleavage studies of two palladium(II) saccharinate complexes with terpyridine". DNA and Cell Biology, 32(4), 165-172. |
Abstract: | The aim of the present study is to investigate DNA binding interactions of two highly cytotoxic palladium(II) saccharinate (sac) complexes with 2,2':6',2 ''-terpyridine (terpy), [Pd(sac)(terpy)](sac)center dot 4H(2)O (1) and [PdCl (terpy)](sac)center dot 2H(2)O (2). UV-Vis spectroscopy, fluorescence spectroscopy, thermal denaturation, viscosity, and gel electrophoresis measurements were employed to study binding properties of the two complexes with Fish Sperm (FS) DNA. The interaction of the palladium(II) complexes with FS-DNA was monitored by hypochromism in the UV-Vis spectra. The binding constants (K-b) obtained from absorption titrations were estimated to be 1.0 (+/- 0.05) x 10(5) M-1 for 1 and 2.0 (+/- 0.22) x 10(5) M-1 for 2. Competitive binding studies with ethidium bromide have shown that both complexes strongly bind to FS-DNA, and the apparent binding constant (K-app) calculated from emission studies was 2.0 x 10(6) M-1 for each complex. Fluorescence Scatchard plots demonstrated that the palladium(II) complexes act as dual-function metallointercalators and bind to FS-DNA by both intercalation and coordination. On addition of the two complexes, significant increases in both viscosity and melting temperature of FS-DNA solutions further support the binding mode of these complexes. Gel electrophoresis assay demonstrates that both complexes efficiently cleavage the supercoiled plasmid 8454: pCMV-VSV-G DNA to its open circular form. |
URI: | https://doi.org/10.1089/dna.2012.1959 https://www.liebertpub.com/doi/10.1089/dna.2012.1959 http://hdl.handle.net/11452/28993 |
ISSN: | 1044-5498 1557-7430 |
Appears in Collections: | Scopus Web of Science |
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