Bu öğeden alıntı yapmak, öğeye bağlanmak için bu tanımlayıcıyı kullanınız: http://hdl.handle.net/11452/26403
Başlık: Analysis of ancient DNA from in vitro grown tissues of 1600-year-old seeds revealed the species as Anagyris foetida
Yazarlar: Özgen, Murat
Özdilek, Aslı
Birsin, Melahat A.
Önde, Sertaç
Kaya, Zeki
Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Arkeoloji Bölümü.
Uludağ Üniversitesi/Ziraat Fakültesi/Tarla Bitkileri Bölümü.
Şahin, Derya
Açıkgöz, Esvet
55343714100
55480264700
Anahtar kelimeler: Plant sciences
Anagyris foetida
Ancient dna
Ancient seeds
Internal transcribed spacer
Seed longevity
Sacred lotus
Germination
L.
Herbarium
Genetics
Samples
Plants
Wood
Yayın Tarihi: Ara-2012
Yayıncı: Cambridge University
Atıf: Özgen, M. vd. (2012). "Analysis of ancient DNA from in vitro grown tissues of 1600-year-old seeds revealed the species as Anagyris foetida". Seed Science Research, 22(4), 279-286.
Özet: Seven ancient seeds, about 1600 years old, were found during an archaeological excavation in Asar Island which is located in south-western Turkey. These seeds were subjected to germination, in vitro callus induction and molecular characterization experiments to test the viability and plant origin of the seeds. Six of the seven seeds had viable seed components (such as cotyledons) and produced callus tissue in Murashige and Skoog medium supplemented with 2mgl(-1) 6-benzylamino purine (BAP), 0.2 mgl(-1) 1-naphthaleneacetic acid (NAA), 20 gl(-1) sucrose, 2mgl(-1) glycine and 7 gl(-1) agar, but the calli from these seeds failed to yield adventitious shoots. DNA samples from callus tissues produced by ancient seeds in vitro were of good quality. The internal transcribed spacer (ITS) region in nuclear DNA (nDNA) of ancient seeds was amplified successfully. The sequences from amplified ITS DNA products of six ancient seeds indicated that their ITS sequences matched those of Anagyris foetida after subjecting them to BLAST searches in international sequence databases (NCBI). A. foetida is a relict species endemic to the Mediterranean region and used as a herbal medicine. We believe that seed characteristics such as the very hard, extremely smooth and shiny testa, toxic anagyrine alkaloid content and their storage in a pot further improved the longevity of these ancient seeds.
URI: https://doi.org/10.1017/S0960258512000207
https://pubag.nal.usda.gov/catalog/5176145
http://hdl.handle.net/11452/26403
ISSN: 0960-2585
1475-2735
Koleksiyonlarda Görünür:Scopus
Web of Science

Bu öğenin dosyaları:
Bu öğeyle ilişkili dosya bulunmamaktadır.


DSpace'deki bütün öğeler, aksi belirtilmedikçe, tüm hakları saklı tutulmak şartıyla telif hakkı ile korunmaktadır.