Please use this identifier to cite or link to this item: http://hdl.handle.net/11452/24672
Full metadata record
DC FieldValueLanguage
dc.contributor.authorButers, Jeroen T. M.-
dc.contributor.authorThibaudon, Michel-
dc.contributor.authorSmith, Matt-
dc.contributor.authorKennedy, Roy-
dc.contributor.authorRantio-Lehtimaki, Auli-
dc.contributor.authorAlbertini, Roberto-
dc.contributor.authorReese, Gerald-
dc.contributor.authorWeber, Bernhard-
dc.contributor.authorGalan, Carmen-
dc.contributor.authorBrandao, Rui-
dc.contributor.authorAntunes, Celia M.-
dc.contributor.authorJaeger, Siegfried-
dc.contributor.authorBerger, Uwe-
dc.contributor.authorGrewling, Lukasz-
dc.contributor.authorJackowiak, Bogdan-
dc.contributor.authorSauliene, Ingrida-
dc.contributor.authorWeichenmeier, Ingrid-
dc.contributor.authorPusch, Gudrun-
dc.contributor.authorSarıoğlu, Hakan-
dc.contributor.authorUeffing, Marius-
dc.contributor.authorBehrendt, Heidrun-
dc.contributor.authorPrank, Marje-
dc.contributor.authorSofiev, Mikhail-
dc.contributor.authorCecchi, Lorenzo-
dc.date.accessioned2022-02-27T19:53:11Z-
dc.date.available2022-02-27T19:53:11Z-
dc.date.issued2012-08-
dc.identifier.citationButers, J. T. M. vd. (2012). "Release of Bet v 1 from birch pollen from 5 European countries. Results from the HIALINE study". Atmospheric Environment, 55, 496-505.en_US
dc.identifier.issn1352-2310-
dc.identifier.issn1873-2844-
dc.identifier.urihttps://doi.org/10.1016/j.atmosenv.2012.01.054-
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S1352231012000751-
dc.identifier.urihttp://hdl.handle.net/11452/24672-
dc.description.abstractExposure to allergens is pivotal in determining sensitization and allergic symptoms in individuals. Pollen grain counts in ambient air have traditionally been assessed to estimate airborne allergen exposure. However, the exact allergen content of ambient air is unknown. We therefore monitored atmospheric concentrations of birch pollen grains and the matched major birch pollen allergen Bet v 1 simultaneously across Europe within the EU-funded project HIALINE (Health Impacts of Airborne Allergen Information Network). Pollen count was assessed with Hirst type pollen traps at 10 I min(-1) at sites in France, United Kingdom, Germany, Italy and Finland. Allergen concentrations in ambient air were sampled at 800 I min(-1) with a Chemvol (R) high-volume cascade impactor equipped with stages PM > 10 mu m, 10 mu m > PM > 2.5 mu m, and in Germany also 2.5 mu m > PM > 0.12 mu m. The major birch pollen allergen Bet v 1 was determined with an allergen specific ELISA. Bet v 1 isoform patterns were analyzed by 2D-SDS-PAGE blots and mass spectrometric identification. Basophil activation was tested in an FC epsilon R1-humanized rat basophil cell line passively sensitized with serum of a birch pollen symptomatic patient. Compared to 10 previous years, 2009 was a representative birch pollen season for all stations. About 90% of the allergen was found in the PM > 10 mu m fraction at all stations. Bet v 1 isoforms pattern did not vary substantially neither during ripening of pollen nor between different geographical locations. The average European allergen release from birch pollen was 3.2 pg Bet v 1/pollen and did not vary much between the European countries. However, in all countries a >10-fold difference in daily allergen release per pollen was measured which could be explained by long-range transport of pollen with a deviating allergen release. Basophil activation by ambient air extracts correlated better with airborne allergen than with pollen concentration. Although Bet v 1 is a mixture of different isoforms, its fingerprint is constant across Europe. Bet v 1 was also exclusively linked to pollen. Pollen from different days varied >10-fold in allergen release. Thus exposure to allergen is inaccurately monitored by only monitoring birch pollen grains. Indeed, a humanized basophil activation test correlated much better with allergen concentrations in ambient air than with pollen count. Monitoring the allergens themselves together with pollen in ambient air might be an improvement in allergen exposure assessment.en_US
dc.description.sponsorshipEuropean Commissionen_US
dc.description.sponsorshipChristine Kühne - Center for Allergy Research and Educationen_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Scienceen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rightsAtıf Gayri Ticari Türetilemez 4.0 Uluslararasıtr_TR
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectEnvironmental sciences & ecologyen_US
dc.subjectMeteorology & atmospheric sciencesen_US
dc.subjectBirchen_US
dc.subjectPollenen_US
dc.subjectBet v 1en_US
dc.subjectExposureen_US
dc.subjectElisaen_US
dc.subjectHialineen_US
dc.subjectHumanen_US
dc.subjectBasophilsen_US
dc.subjectEuropeen_US
dc.subjectIsoformsen_US
dc.subjectOlea-europaea pollenen_US
dc.subjectMast-cell linesen_US
dc.subjectAllergic rhinitisen_US
dc.subjectCreate projecten_US
dc.subjectClimate-changeen_US
dc.subjectPrevalenceen_US
dc.subjectAirborneen_US
dc.subjectAsthmaen_US
dc.subjectSensitizationen_US
dc.subjectParticlesen_US
dc.subjectFinlanden_US
dc.subjectFranceen_US
dc.subjectGermanyen_US
dc.subjectItalyen_US
dc.subjectUnited Kingdomen_US
dc.subjectRattusen_US
dc.subjectAntigensen_US
dc.subjectCell cultureen_US
dc.subjectInformation servicesen_US
dc.subjectMeteorological instrumentsen_US
dc.subjectAerosolen_US
dc.subjectAllergyen_US
dc.subjectAmbient airen_US
dc.subjectAtmospheric pollutionen_US
dc.subjectConcentration (composition)en_US
dc.subjectDeciduous treeen_US
dc.subjectEuropean unionen_US
dc.subjectHealth impacten_US
dc.subjectPollution exposureen_US
dc.subjectPollution monitoringen_US
dc.subjectSymptomen_US
dc.subjectAllergiesen_US
dc.titleRelease of Bet v 1 from birch pollen from 5 European countries. Results from the HIALINE studyen_US
dc.typeArticleen_US
dc.identifier.wos000306870400063tr_TR
dc.identifier.scopus2-s2.0-84860220045tr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.contributor.departmentUludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.tr_TR
dc.contributor.orcid0000-0003-4925-8902tr_TR
dc.identifier.startpage496tr_TR
dc.identifier.endpage505tr_TR
dc.identifier.volume55tr_TR
dc.relation.journalAtmospheric Environmenten_US
dc.contributor.buuauthorÇelenk, Sevcan-
dc.contributor.researcheridK-2981-2012tr_TR
dc.relation.collaborationYurt dışıtr_TR
dc.relation.collaborationSanayitr_TR
dc.subject.wosEnvironmental sciencesen_US
dc.subject.wosMeteorology & atmospheric sciencesen_US
dc.indexed.wosSCIEen_US
dc.indexed.scopusScopusen_US
dc.wos.quartileQ1en_US
dc.contributor.scopusid24170598000tr_TR
dc.subject.scopusRagweed; Ambrosia Artemisiifolia; Pollenen_US
dc.subject.emtreeBet v 1en_US
dc.subject.emtreePollen antigenen_US
dc.subject.emtreeUnclassified drugen_US
dc.subject.emtreeAmbient airen_US
dc.subject.emtreeAnimal cellen_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeBasophilen_US
dc.subject.emtreeBirchen_US
dc.subject.emtreeCell activationen_US
dc.subject.emtreeConcentration (parameters)en_US
dc.subject.emtreeControlled studyen_US
dc.subject.emtreeEnzyme linked immunosorbent assayen_US
dc.subject.emtreeFinlanden_US
dc.subject.emtreeFranceen_US
dc.subject.emtreeGermanyen_US
dc.subject.emtreeItalyen_US
dc.subject.emtreeMass spectrometryen_US
dc.subject.emtreeNonhumanen_US
dc.subject.emtreePollenen_US
dc.subject.emtreePolyacrylamide gel electrophoresisen_US
dc.subject.emtreePriority journalen_US
dc.subject.emtreeRaten_US
dc.subject.emtreeUnited Kingdomen_US
Appears in Collections:Scopus
Web of Science

Files in This Item:
File Description SizeFormat 
Çelenk_vd_2012.pdf1.23 MBAdobe PDFThumbnail
View/Open


This item is licensed under a Creative Commons License Creative Commons